Composite

Part:BBa_K2248001:Design

Designed by: Judy Nguyen   Group: iGEM17_Cadets2Vets   (2017-09-16)


PcArsrR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 198
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PcArsR is the unregulated part of our circuit, so we needed to make sure that it was at the 5' end of our 2-part circuit. This allows it to be produced as a regulator for a downstream sequence. Because we had to clone another composite part behind PcArsR, we placed the SalI linker at the 3' end of it.


Source

The parts are E. coli parts already in the registry.

References